The Chromatrap chromatin immunoprecipitation (ChIP) assay range has been added to the manufacturer’s portfolio of products for epigenetics. Developed in conjunction with the Department of Life Sciences at the UK’s University of Swansea, the new assay uses a solid-phase porous polymer functionalized with protein A, allowing the chromatin capture to be more efficient than bead based methods. Manufacturer says the spin column approach offers significant advantages compared to methods based on sepharose or magnetic beads, which involve many steps of separation, pipetting and re-suspension. Independent tests with Chromatrap demonstrate levels of DNA pull down up to 25 times higher than with traditional bead based procedures, excellent DNA enrichment with signal-to-noise ratios typically 2 to 3 times better than competing procedures and top performance even with samples as small as 50ng or as much as 3000ng chromatin per immunoprecipitation. Porvair Sciences
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