Ensuring that autosamplers extract even the smallest sample volumes every time, these Limited Volume Inserts with bottom and top springs work without damaging injection needles or breaking sample containers. The inserts are preassembled with polymer bottom springs or the Top Spring, which act as shock absorbers to protect sample needles from bottoming out during sample extraction. The precision internal point design permits maximum sample withdrawal. They allow chemists to analyze smaller sample volumes in standard size vials that are compatible with autosamplers. Composed of borosilicate glass or polypropylene, they are available with bottom or top springs in 0.050mL, 0.100mL and 0.250mL sample volumes.