Vacuum-operated and designed to eliminate spills and contamination, the Autofil System features an ergonomically designed bottle and a stable pedestal that enables hands-free operation. Suitable for the preparation of buffers, tissue culture media, microbiological media and other biological fluids, the complete system consists of a disposable Autofil filter funnel, a disposable SECUREgrasp bottle, and the Quick-Connect pedestal. The disposable components of the system are sold pre-sterilized. The filter funnel is also available as a stand-alone filter that is compatible with any 45 thread bottle. A surfactant-free, low-protein-binding polyethersulfone (PES) membrane is incorporated in the filter funnel and is available in both 0.22 μm and 0.45 μm pore sizes.