Traditional immunohistochemistry (IHC) methods can be subject to process variability. For example, the process typically requires a lot of manual slide handling, as well as the use of pap pens and pipettes. It is therefore harder to apply consistent conditions across slides, which may negatively affect research results.
The SNAP i.d. 2.0 Protein Detection System for Immunohistochemistry (IHC) represents an advancement in the IHC workflow. The power of IHC lies in its capacity to localize antigens within tissue samples, thereby identifying the cell types and subcellular compartments in which antigens are located. This is particularly important for heterogeneous tissues (i.e., tissues made up of many different cell types, such as the brain and tumours), for which other antigen detection methods do not reveal spatial information.
To overcome the problem of process variability, the SNAP i.d. 2.0 system systemizes the handling of multiple slides. It decreases slide-handling time and enables parallel processing of up to 24 slides at once, reducing slide-to-slide process variation without incurring the costs of automation. In addition, the system speeds wash steps and allows antibodies to be recovered and reused, saving researchers valuable time and resources.
The system produces robust and consistent staining, without causing tissue degradation or blotchy artifacts. It also offers the flexibility for researchers to perform Western blotting and IHC experiments in parallel, facilitating use of the system by multiple users at once, and also makes it possible to simultaneously assess relative abundance of an antigen (by Western blotting) and spatial distribution (by immunohistochemistry).
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