While serum-free media is well established, StemSpan ACF is a new medium for the culture and expansion of hematopoietic stem cells that is free of animal and human proteins and only contains recombinant and synthetic components. It allows researchers to study hematopoiesis and cell therapy development without concerns of transmission of adventitious agents from plasma derived components, or lack of consistency between different lots of human or bovine proteins in other complete media formulations. It supports equivalent total nuclear cell and CD34+ cell expansion as the existing highest performance serum-free medium on the market. It also supports monocyte to dendritic cell differentiation and might be used by researchers to develop and optimize their own culture systems to generate large numbers of other mature blood cells.
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