Designed to enable fast and cost-effective Next-Generation Sequencing (NGS), these small RNA and mRNA sample library construction kits reduce NGS workflow complexity, shorten sample library preparation time and labour, and minimise errors by eliminating extra steps and optimising the experiment protocol. With input RNA requirement as low as 0.5ng, these kits can be used for the sample preparation from rare and difficult to obtain sources, such as the pathological samples. They can also be used for the situations where the samples are degraded as with formalin fixed paraffin embedded (FFPE) tissue samples.