Including modules for the study of neuronal morphology and fiber structures, support for targeted illumination devices, and a high-speed image acquisition mode the MetaMorph NX Microscopy Automation and Image Analysis Software now simplifies the challenging, meticulous tasks associated with neuron research and makes sense of complex, interwoven fiber structures. Added support for targeted illumination devices enables fast synchronization with regions of interest or an exact position in the image, and the new high-speed image acquisition mode allows researchers to record unlimited numbers of rapid changes in cellular function and behavior. The software provides a single tool for researchers to use images of any size to rapidly produce quantitative and comparative descriptions of neuronal anatomy.