Automated system performs western blots with high sensitivity and low background noise. The BlotCycler uses a simple and elegant design to perform up to 12 blots simultaneously. Researchers run samples using one or two different protocols at the same time, and continue using the same industry standard reagents and protocols.
The system automates blocking, primary and secondary antibody incubation, and all washing steps. The system is equipped with up to 6 trays, 3 trays on each side that can process 6 different primary and secondary antibodies. Researchers fill containers with their antibodies and buffer solutions, add the blotting membranes, and set the blocking time from 10 minutes to 48 hours. The system maintains a shaking motion for consistent processing. When blots are removed the cleaning protocol employs a special washing routine for thorough cleaning, contributing to assay reproducibility.
By reducing variables from slight variations in fluid delivery, timing and washing steps, the system allows researchers to achieve standardization with a repeatable automated process. The manufacturer says it guarantees users will consistently get better western blots or get a full refund. Precision Biosystems